Bacterial DNA ligases
نویسندگان
چکیده
منابع مشابه
19 DNA Ligases
DNA ligases are Mg++-dependent enzymes that catalyze the formation of phosphodiester bonds at single-strand breaks in double-stranded DNA (for review and main references up to 1992, see Engler and Richardson 1982; Lindahl and Barnes 1992). The first step in the reaction is the formation of a covalent enzyme/adenylate intermediate. DNA ligases from eukaryotes, archaea, and viruses employ ATP as ...
متن کاملBase-modified NAD and AMP derivatives and their activity against bacterial DNA ligases.
We report the chemical synthesis and conformational analysis of a collection of 2-, 6- and 8-substituted derivatives of β-NAD(+) and AMP, and their biochemical evaluation against NAD(+)-dependent DNA ligases from Escherichia coli and Mycobacterium tuberculosis. Bacterial DNA ligases are validated anti-microbial targets, and new strategies for their inhibition are therefore of considerable scien...
متن کاملMitochondrial DNA ligases of Trypanosoma brucei.
The mitochondrial DNA of Trypanosoma brucei, termed kinetoplast DNA or kDNA, consists of thousands of minicircles and a small number of maxicircles catenated into a single network organized as a nucleoprotein disk at the base of the flagellum. Minicircles are replicated free of the network but still contain nicks and gaps after rejoining to the network. Covalent closure of remaining discontinui...
متن کاملCRL Ubiquitin Ligases and DNA Damage Response
Cullin/RING ubiquitin ligases (CRL) comprise the largest subfamily of ubiquitin ligases. CRLs are involved in cell cycle regulation, DNA replication, DNA damage response (DDR), development, immune response, transcriptional regulation, circadian rhythm, viral infection, and protein quality control. One of the main functions of CRLs is to regulate the DDR, a fundamental signaling cascade that mai...
متن کاملDNA ligases ensure fidelity by interrogating minor groove contacts.
DNA ligases, found in both prokaryotes and eukaryotes, covalently link the 3'-hydroxyl and 5'-phosphate ends of duplex DNA segments. This reaction represents a completion step for DNA replication, repair and recombination. It is well established that ligases are sensitive to mispairs present on the 3' side of the ligase junction, but tolerant of mispairs on the 5' side. While such discriminatio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2001
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.2001.02479.x